Aquisalimonas lutea sp. nov., a moderately halophilic bacterium from a salternby C. Infante-Dominguez, C. Sanchez-Porro, A. Ventosa

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY

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Year
2015
DOI
10.1099/ijs.0.000106
Subject
Ecology, Evolution, Behavior and Systematics / Microbiology

Text

International Journal of Systematic and Evolutionary Microbiology

Aquisalimonas lutea sp. nov., a moderately halophilic bacterium from a saltern --Manuscript Draft-Manuscript Number: IJS-D-14-00569R1

Full Title: Aquisalimonas lutea sp. nov., a moderately halophilic bacterium from a saltern

Short Title: Aquisalimonas lutea sp. nov.

Article Type: Note

Section/Category: New taxa - Proteobacteria

Corresponding Author: Antonio Ventosa

University of Sevilla

Sevilla, SPAIN

First Author: Carmen Infante-Dominguez

Order of Authors: Carmen Infante-Dominguez

Cristina Sánchez-Porro

Antonio Ventosa

Manuscript Region of Origin: SPAIN

Abstract: A yellow-pigmented, motile, Gram-stain-negative, moderately halophilic and strictly aerobic bacterium, designated BA42AL-1T was isolated from water of a saltern of

Santa Pola, Alicante, Spain. Strain BA42AL-1T grew in media containing 5-20 % (w/v) salts and optimally at 7.5 % (w/v) salts. It grew between pH 6.0 and 9.0 (optimally at pH 7.5) and at 14-45 ºC (optimally at 37 ºC). Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain BA42AL-1T is a member of the genus Aquisalimonas. The closest relatives to this strain were

Aquisalimonas halophila YIM 95345T and Aquisalimonas asiatica CG12T with sequence similarities of 99.4 % and 97.0 %, respectively. DNA-DNA hybridization between the novel isolate and the type strain of the phylogenetically related species

Aquisalimonas halophila YIM 95345T revealed a relatedness of 54 %. The major fatty acids of strain BA42AL-1T were C18:1 ω6c/C18:1 ω7c, C19:0 cyclo ω8c and C16:0, and lower contents of C12:0 and C18:0. The polar lipid pattern of strain BA42AL-1T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, two glycolipids, a lipid and four unknown phospholipids. The G + C content of the genomic DNA of this strain was 65.0 mol%. Based on the DNA-DNA hybridization, phenotypic, chemotaxonomic and phylogenetic data presented in this study, the strain BA42AL-1T is proposed as a novel species of the genus Aquisalimonas, for which the name Aquisalimonas lutea sp. nov. is suggested. The type strain is BA42AL-1T (= CCM 8472T = CECT 8326T = LMG 27614T).

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IJSEM Papers in Press. Published February 9, 2015 as doi:10.1099/ijs.0.000106 1 1

Aquisalimonas lutea sp. nov., a moderately halophilic bacterium from a saltern 2 3

Carmen Infante-Domínguez, Cristina Sánchez-Porro and Antonio Ventosa 4 5 6

Department of Microbiology and Parasitology, Faculty of Pharmacy, University of 7

Sevilla, 41012 Sevilla, Spain. 8 9 10

Running title: Aquisalimonas lutea sp. nov. 11

Subject category: New taxa, Proteobacteria 12 13

Author for correspondence: Antonio Ventosa, Department of Microbiology and 14

Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain. E.-mail: 15 ventosa@us.es 16 17

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of 18 strain BA42AL-1

T is HF954114. 19 20

Two supplementary figures and a supplementary table are available with the online 21

Supplementary Material. 22 23

Manuscript Including References (Word document)

Click here to download Manuscript Including References (Word document): Aquisalimonas lutea revised.doc 2 24

A yellow-pigmented, motile, Gram-stain-negative, moderately halophilic and 25 strictly aerobic bacterium, designated BA42AL-1

T was isolated from water of a 26 saltern of Santa Pola, Alicante, Spain. Strain BA42AL-1

T grew in media containing 27 5-20 % (w/v) salts and optimally at 7.5 % (w/v) salts. It grew between pH 6.0 and 28 9.0 (optimally at pH 7.5) and at 14-45 ºC (optimally at 37 ºC). Phylogenetic analysis 29 based on the comparison of 16S rRNA gene sequences revealed that strain 30

BA42AL-1

T is a member of the genus Aquisalimonas. The closest relatives to this 31 strain were Aquisalimonas halophila YIM 95345

T and Aquisalimonas asiatica 32

CG12

T with sequence similarities of 99.4 % and 97.0 %, respectively. DNA-DNA 33 hybridization between the novel isolate and the type strain of the phylogenetically 34 related species Aquisalimonas halophila YIM 95345

T revealed a relatedness of 54 35 %. The major fatty acids of strain BA42AL-1

T were C18:1 ω6c/C18:1 ω7c, C19:0 cyclo 36 ω8c and C16:0, and lower contents of C12:0 and C18:0. The polar lipid pattern of 37 strain BA42AL-1

T consisted of diphosphatidylglycerol, phosphatidylglycerol, 38 phosphatidylmethylethanolamine, phosphatidylcholine, two glycolipids, a lipid and 39 four unknown phospholipids. The G + C content of the genomic DNA of this strain 40 was 65.0 mol%. Based on the DNA-DNA hybridization, phenotypic, 41 chemotaxonomic and phylogenetic data presented in this study, the strain 42

BA42AL-1

T is proposed as a novel species of the genus Aquisalimonas, for which 43 the name Aquisalimonas lutea sp. nov. is suggested. The type strain is BA42AL-1

T 44 (= CCM 8472

T = CECT 8326

T = LMG 27614

T ). 45 46 3

The genus Aquisalimonas (family Ectothiorhodospiraceae, class 47

Gammaproteobacteria) was originally proposed by Márquez et al. (2007). Currently, 48 this genus includes two species: Aquisalimonas asiatica (Márquez et al., 2007), isolated 49 from the alkaline, saline lake Chagannor in Inner Mongolia Autonomous Region, 50

China, as the type species of the genus, and Aquisalimonas halophila (Zhang et al., 51 2014), isolated from a soil sample of a salt mine in Yunnan, China. The genus 52

Aquisalimonas includes Gram-stain-negative, motile and rod-shaped cells arranged as 53 single, in pairs or small chains. They are catalase and oxidase positive, and moderately 54 halophilic bacteria. The major cellular fatty acids are C18:1 ω7c, C19:0 cyclo ω8c, C16:0, 55

C18:0 and C12:0. The polar lipids of species of this genus are diphosphatidylglycerol, 56 phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, 57 phosphatidylinositol, and some unidentified aminolipids and phospholipids. The DNA 58 content G+C content is 62.1 to 64.0 mol% (Márquez et al., 2007; Zhang et al., 2014). 59