Autofluorescence: Biological functions and technical applicationsby José Ignacio García-Plazaola, Beatriz Fernández-Marín, Stephen O. Duke, Antonio Hernández, Fernando López-Arbeloa, José María Becerril

Plant Science

About

Year
2015
DOI
10.1016/j.plantsci.2015.03.010
Subject
Agronomy and Crop Science / Plant Science / Genetics

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Text

Plant Science 236 (2015) 136–145

Contents lists available at ScienceDirect

Plant Science j ourna l ho me pa g e: www.elsev ier .com/ locate /p lantsc i

Review

Autofluorescence: Biological functions and techn

José Igna

Antonio a Dpto Biología b Institute of Bo c Natural Produ d Dpto Química a r t i c l

Article history:

Received 3 De

Received in re

Accepted 14 M

Available onlin

Keywords:

Biocommunic

Emission

Fluorescence

Fluorophore

Green fluorescent protein of some metabolites in plants and cells, complementing and overcoming some of the limitations of the use of fluorescent proteins and dyes to probe plant physiology and biochemistry. Some fluorophores are influenced by environmental interactions, allowing fluorescence to be also used as a specific stress indicator. © 2015 Elsevier Ireland Ltd. All rights reserved.

Contents 1. Fluore 1.1. 1.2. 2. Optica 3. Are th 3.1. 3.2. 4. Autofl 4.1. 4.2. 4.3. 5. Concl

Ackno

Refer

Abbreviati protoporphyri ∗ Correspon

E-mail add http://dx.doi.o 0168-9452/© scence, the basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136

Basic aspects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136

Factors affecting fluorescence quantum efficiency and spectrum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137 l properties of plant tissues and fluorophores . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 138 ere advantages of fluorescence to plants? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140

Fluorescent compounds as cues in biocommunication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140

Conversion of harmful UV radiation into harmless photosynthetically active wavelengths . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141 uorescence in biological studies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 142

Up to cell level: use of autofluorescence for biochemical, physiological and anatomical purposes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 142

Quality and stress assessments: from molecules to satellites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 142

Technical limitations and advances in autofluorescence measurements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143 uding remarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143 wledgements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144 ences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144 ons: F, fluorophore; FRET, Förster resonance energy transfer; ProtoIX, n IX; Q, quencher. ding author. Tel.: +34 946015319; fax: +34 946013500. ress: joseignacio.garcia@ehu.es (J.I. García-Plazaola). 1. Fluorescence, the basics 1.1. Basic aspects

Luminescence refers to the spontaneous emission of light by atoms or molecules as a consequence of the deactivation from an electronic excited state to the ground state. If the radiative rg/10.1016/j.plantsci.2015.03.010 2015 Elsevier Ireland Ltd. All rights reserved.cio García-Plazaolaa,∗, Beatriz Fernández-Marína,b, Stephen O. Dukec,

Hernándeza, Fernando López-Arbeload, José María Becerril a

Vegetal y Ecología, Universidad del País Vasco (UPV/EHU), Apdo. 644, 48080 Bilbao, Spain tany and Center for Molecular Biosciences Innsbruck, University of Innsbruck, Sternwartestraße 15, A-6020 Innsbruck, Austria cts Utilization Research Unit, USDA, ARS, University of Mississippi, University, MS 38677, USA

Física, Universidad del País Vasco (UPV/EHU), Apdo. 644, 48080 Bilbao, Spain e i n f o cember 2014 vised form 13 March 2015 arch 2015 e 21 March 2015 ation a b s t r a c t