Characterization and mapping of LanrBo: a locus conferring anthracnose resistance in narrow-leafed lupin (Lupinus angustifolius L.)by Kristin Fischer, Regine Dieterich, Matthew N. Nelson, Lars G. Kamphuis, Karam B. Singh, Björn Rotter, Nicolas Krezdorn, Peter Winter, Peter Wehling, Brigitte Ruge-Wehling

Theor Appl Genet

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Year
2015
DOI
10.1007/s00122-015-2572-3
Subject
Biotechnology / Agronomy and Crop Science / Genetics

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Theor Appl Genet (2015) 128:2121–2130

DOI 10.1007/s00122-015-2572-3

ORIGINAL ARTICLE

Characterization and mapping of LanrBo: a locus conferring anthracnose resistance in narrow‑leafed lupin (Lupinus angustifolius L.)

Kristin Fischer1 · Regine Dieterich2 · Matthew N. Nelson3,4 · Lars G. Kamphuis4,5 ·

Karam B. Singh4,5 · Björn Rotter6 · Nicolas Krezdorn6 · Peter Winter6 ·

Peter Wehling1 · Brigitte Ruge‑Wehling1

Received: 23 February 2015 / Accepted: 23 June 2015 / Published online: 14 July 2015 © Springer-Verlag Berlin Heidelberg 2015 data obtained with inoculated resistant vs. susceptible lupin entries as well as EST-sequence information from the model genome Lotus japonicus, additional SNP and

EST markers linked to LanrBo were derived. A bracket of two LanrBo-flanking markers allows for precise markerassisted selection of the novel resistance gene in narrowleafed lupin breeding programs.

Introduction

Recent developments in the EU Common Agricultural

Policy, as well as national policies, to open up ecosystem services provided by domestic legumes, have called attention in so far underutilized grain legumes in European agriculture.

Among these, sweet narrow-leafed lupin (Lupinus angustifolius L.) offers a highly valuable protein source for both feed and food purposes. Moreover lupin cultivation provides benefits for sustainable agriculture as they are able to mobilize soil phosphorous and fix atmospheric nitrogen; hence they offer attractive options to provide a more flexible crop rotation (Lambers et al. 2013).

Present acreage of sweet lupins in Germany amounts to 21,400 ha (DESTATIS 2014), which although still quite low, represents a 23 % increase as compared to 2013.

Reflecting the lupin production worldwide in 2013, 58.4 % took place in Australia and Oceania, followed by Europe with 32 % with the remaining 9.6 % lupin production happened in Africa and America. Being the most important lupin producer worldwide, acreage of lupins in Australia and Oceania amounts to 450,200 ha (FAOSTAT 2014).

In Germany, narrow-leafed lupin largely displaced white and yellow lupins (L. albus L., L. luteus L.) in the midnineties of the last century because of its somewhat higher

Abstract

Key message A novel and highly effective source of anthracnose resistance in narrow‑leafed lupin was iden‑ tified. Resistance was shown to be governed by a single dominant locus. Molecular markers have been devel‑ oped, which can be used for selecting resistant geno‑ types in lupin breeding.

Abstract A screening for anthracnose resistance of a set of plant genetic resources of narrow-leafed lupin (Lupi‑ nus angustifolius L.) identified the breeding line Bo7212 as being highly resistant to anthracnose (Colletotrichum lupini). Segregation analysis indicated that the resistance of Bo7212 is inherited by a single dominant locus. The corresponding resistance gene was given the designation

LanrBo. Previously published molecular anchor markers allowed us to locate LanrBo on linkage group NLL-11 of narrow-leafed lupin. Using information from RNAseq

Communicated by D. A. Lightfoot.

Electronic supplementary material The online version of this article (doi:10.1007/s00122-015-2572-3) contains supplementary material, which is available to authorized users. * Kristin Fischer kristin.fischer@jki.bund.de 1 Julius Kühn-Institut, Institute for Breeding Research on Agricultural Crops, Groß Lüsewitz, Germany 2 Saatzucht Steinach GmbH & Co KG, Bocksee, Germany 3 School of Plant Biology, The University of Western Australia,

Crawley, Australia 4 The UWA Institute of Agriculture, The University of Western

Australia, Crawley, Australia 5 CSIRO, Agriculture Flagship, Wembley, Australia 6 GenXPro GmbH, Frankfurt/Main, Germany 2122 Theor Appl Genet (2015) 128:2121–2130 1 3 tolerance to the fungus Colletotrichum lupini, the causal agent of anthracnose (Wolko et al. 2011). However, most if not all narrow-leafed lupin cultivars adapted to agricultural conditions in Central Europe lack strong resistance to the fungus. Being a seed-transmitted disease (Gondran et al. 1994), anthracnose continues to pose a latent threat to the cultivation of narrow-leafed lupin, not only in Germany but also worldwide (Paulitz 1995; Reed et al. 1996; Sweetingham et al. 1995), thereby emphasizing the necessity of resistant cultivars.

In Australia, after dealing with a disease spread in the mid-1990s, the anthracnose-resistant cvs. ‘Mandelup’ and ‘Tanjil’ were released, thereby exemplifying that genes for anthracnose resistance exist in L. angustifolius and that resistance breeding provides an option to fight the disease (Yang et al. 2004, 2008). Resistance in cv. ‘Tanjil’ is inherited by a single dominant gene named Lanr1 (Yang et al. 2004), which is located on linkage group

NLL-11 (Nelson et al. 2010). This resistance gene can be tracked in breeding programs by use of closely linked codominant molecular markers (Yang et al. 2012; You et al. 2005).

When grown under local German growing conditions, cvs. ‘Tanjil’ and ‘Mandelup’ prove to be less susceptible than standard cultivars; however they still become infested by the pathogen to considerable extents (Ruge-Wehling et al. 2009; this paper). Thus, provision of additional genes for anthracnose resistance appears desirable to lupin breeders.

In the present study, we performed a screening of cultivars, breeding lines and genebank accessions and assessed their susceptibility to anthracnose in the greenhouse and under field conditions at diverse locations. We report on a novel anthracnose-resistance gene, LanrBo, and the development of molecular markers using existing and novel resources, which may be used to select for this gene in breeding programs.

Materials and methods

Material

L. angustifolius accessions

A set of 13 L. angustifolius cultivars (‘Arabella’, ‘Bolivio’, ‘Bora’, ‘Bordako’, ‘Boregine’, ‘Boruta’, ‘Borweta’, ‘Haagena’, ‘Haags Blaue’, ‘Mandelup’, ‘Polonez’, ‘Tanjil’, and ‘Vitabor’), 15 breeding lines from the seed breeding company Saatzucht Steinach GmbH & Co KG, Bocksee,